Extension of a de novo TIM barrel with a rationally designed secondary structure element

The ability to construct novel enzymes is a major aim in de novo protein design. A popular enzyme fold for design attempts is the TIM barrel. This fold is a common topology for enzymes and can harbor many diverse reactions. The recent de novo design of a four-fold symmetric TIM barrel provides a well understood minimal scaffold for potential enzyme designs. Here we explore opportunities to extend and diversify this scaffold by adding a short de novo helix on top of the barrel. Due to the size of the protein, we developed a design pipeline based on computational ab initio folding that solves a less complex sub-problem focused around the helix and its vicinity and adapt it to the entire protein. We provide biochemical characterization and a high-resolution X-ray structure for one variant and compare it to our design model. The successful extension of this robust TIM-barrel scaffold opens opportunities to diversify it towards more pocket like arrangements and as such can be considered a building block for future design of binding or catalytic sites

Slow Logistics – eine simulationsgestützte Analyse der ökonomischen und ökologischen Potentiale der Sendungsbündelung

Next Day, Next Morning, Same Day, Same Hour – customers are getting their orders faster and faster. Today high speed delivery based on accelerated logistical processes is a key competitive factor in distribution logistic, not only in the B2C-Sector. This speed, however, necessitate high use and waste of resources. It thus counteracts current efforts towards more sustainability in value-added processes. It also raises the question of whether speed or rather delivery reliability and sustainable using of resources will be the decisive criteria for customers in the future. Slow Logistics bears on these arguments. This concept breaks with the paradigm of speed in logistics without, however, impairing the economic efficiency. On the contrary, the conscious exploitation of existing time windows in logistical processes in product-specific supply chains combines the economic and social-ecological value added, so that robust systems will be created based on the accepted slowdown. This dissertation developed as first the theoretically fundament of Slow Logistics and describes detailed possible practical implementations. In addition, this book focuses on shipment consolidation as a central method of slow logistics. A comprehensive simulation study evaluate and analysis different strategies in the field of shipment consolidation to close some major research gaps and thereby generating valuable results and recommendations for implementations in business practice

Windenergie-Nutzung

Innerhalb des Gesamtrahmens des Projektes "Klimaverträgliche Energieversorgung in Baden-Württemberg" ist es das Ziel des Gutachtens über die Windenergienutzung, die Möglichkeiten und Grenzen einer windtechnischen Stromerzeugung in Baden-Württemberg darzustellen und zu diskutieren. Gleichzeitig sollen die notwendigen technischen, ökonomischen, ökologischen und sonstigen Eckdaten für die in der zweiten Projektphase geplante Szenarioanalyse, deren Ausgangspunkt die politische Orientierung der CO2-Emissionsminderung um 30% im Zeitraum von 1987 bis 2005 ist, zur Verfügung gestellt werden

Logistics Management - Challanges, Opportunities & Solutions

Gerade in wirtschaftlich schwierigen Zeiten gilt es, die Zukunftsfähigkeit von Unternehmen und ganzen Wertschöpfungsketten zu erhalten, indem die Herausforderungen in der Logistik und dem Supply Chain Management aufgegriffen, Chancen herausgearbeitet und konkrete Lösungen ermittelt werden. Dieser Tagungsband ist Teil einer dreibändigen Reihe, welche die Ergebnisse der Konferenz "Logistikmanagement 2011" der Wissenschaftlichen Kommission Logistik im Verband der Hochschullehrer für Betriebswirtschaft e.V. (VHB) dokumentiert, die an der Otto-Friedrich-Universität in Bamberg stattfand. Die ausgewählten Beiträge präsentieren den gegenwärtigen Forschungsstand der betriebswirtschaftlichen Logistik und des Supply Chain Managements. Es werden aktuelle und innovative Konzepte, Theorien und Lösungsansätze in Logistik und Supply Chain Management eindrucksvoll dargestellt. Das Buch wendet sich an Dozenten und Studenten der Betriebswirtschaftslehre mit den Schwerpunkten Supply Chain Management, Logistik und Operations Management sowie an Praktiker, die in diesen Bereichen tätig sind

SARS-CoV-2 antibody prevalence among homeless people and shelter workers in Denmark:a nationwide cross-sectional study

BACKGROUND: People experiencing homelessness (PEH) and associated shelter workers may be at higher risk of infection with “Severe acute respiratory syndrome coronavirus 2” (SARS-CoV-2). The aim of this study was to determine the prevalence of SARS-CoV-2 among PEH and shelter workers in Denmark. DESIGN AND METHODS: In November 2020, we conducted a nationwide cross-sectional seroprevalence study among PEH and shelter workers at 21 recruitment sites in Denmark. The assessment included a point-of-care test for antibodies against SARS-CoV-2, followed by a questionnaire. The seroprevalence was compared to that of geographically matched blood donors considered as a proxy for the background population, tested using a total Ig ELISA assay. RESULTS: We included 827 participants in the study, of whom 819 provided their SARS-CoV-2 antibody results. Of those, 628 were PEH (median age 50.8 (IQR 40.9–59.1) years, 35.5% female) and 191 were shelter workers (median age 46.6 (IQR 36.1–55.0) years and 74.5% female). The overall seroprevalence was 6.7% and was similar among PEH and shelter workers (6.8% vs 6.3%, p = 0.87); and 12.2% among all participants who engaged in sex work. The overall participant seroprevalence was significantly higher than that of the background population (2.9%, p < 0.001). When combining all participants who reported sex work or were recruited at designated safe havens, we found a significantly increased risk of seropositivity compared to other participants (OR 2.23, 95%CI 1.06–4.43, p = 0.02). Seropositive and seronegative participants reported a similar presence of at least one SARS-CoV-2 associated symptom (49% and 54%, respectively). INTERPRETATIONS: The prevalence of SARS-CoV-2 antibodies was more than twice as high among PEH and associated shelter workers, compared to the background population. These results could be taken into consideration when deciding in which phase PEH are eligible for a vaccine, as part of the Danish national SARS-CoV-2 vaccination program rollout. FUNDING: TrygFonden and HelseFonden. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12889-022-13642-7